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HPLC and UHPLC for Practicing Scientists 1 and 2: Fundamentals, Method Development, and Troubleshooting
November 17 and 18; 8:30am – 5:00pm
Instructor: Michael Dong, MWD Consulting
Course Description:
This intermediate course will provide the analytical scientist with a clearer understanding and a solid working knowledge of the concepts, instrumentation, columns, applications, and best practices in method development and troubleshooting of HPLC and UHPLC (ultra-high-pressure liquid chromatography). The focus is on pharmaceutical analysis of small molecule drugs. This course can also be taken as Day/Part 1 only or Day/Part 2 only. Click here for more details.
Practical LC-MS/MS Method Development & Sample Preparation
November 18 and 19; 8:30am – 5:00pm
Instructor: Perry Wang, LC-MS Technical Expert
Course Description:
This two-day course offers practical training for practical scientists. It will take the participants step‐by‐step through the concepts and techniques to develop LC‐MS methods. The emphasis is on practical issues associated with developing LC‐MS methods for small molecules. It also emphasizes problem‐solving skills with examples encountered in the pharmaceutical industry and other fields. This course will provide the participants with an updated overview and a solid working knowledge of LC‐MS. The participants will learn useful theoretical concepts, instrumental fundamentals and operating principles, column basics and selection guides, and key applications. After this course, the participants will be able to independently develop their own LC‐MS methods. New technologies and techniques, such as monolithic chromatography and hydrophilic interaction liquid chromatography will be presented. Click here for more details.
November 20; 8:30am – 5:00pm
Instructor: Perry Wang, LC-MS Technical Expert
Course Description:
This one-day course is designed to offer practical training for the practicing scientists in the analytical field. Validation of analytical methods is crucial for the successful conduct of nonclinical, biopharmaceutics, and clinical pharmacology studies. This short course will focus on the FDA’s Bioanalytical Method Validation – Guidance for Industry published in May 2018 using LC-MS/MS. It will take the participants step-by-step through the concepts and techniques to validate bioanalytical methods. The emphasis is on practical issues associated with the bioanalytical method validation. The participants will learn the essence of the method validation. After this course, the participants will be able to independently validate their own bioanalytical methods. How to apply the validated methods for routine assay and some typical case studies will be presented as well. Click here for more details.
HPLC Operating Modes Primer: Reversed-Phase & Other Options
November 20; 8:30am – 5:00pm
Instructors: Merlin Bicking, ACCTA, Inc.
Course Description:
Almost every LC lab operates in reversed-phase (RP) mode, but many users are not taking advantage of the full capabilities in this separation technique. Improving methods and troubleshooting problems are easier if you understand the technique, and tremendous improvements in selectivity, efficiency, and/or throughput are possible with only modest modifications. This operations-based one-day primer will provide an overview on the features that allow an RP application to succeed and answer the questions that most users have. We will focus on practice, not theory, providing simple rules for improving your methods to reduce solvent usage or time (or both). Also, not every separation problem can be solved with a C18 column. If C18 does not work for you, you have many other options. We will show you those other options using real-world case studies. Click here for more details.
Chromatographic Methods of Analysis of Oligonucleotides, siRNA, and mRNA
November 18: 8:30am - 5:00pm
Instructor: Martin Gilar, Waters Corp.
Course Description:
We describe development of liquid chromatography (LC) methods for analysis of nucleic acid therapeutic compounds. Ion-pair reversed-phase LC (IP RP LC) or hydrophilic interaction chromatography (HILIC) are method compatible with mass spectrometry (MS) detection. Size exclusion chromatography (SEC) and ion-exchange chromatography (IEC) are useful with simple UV detection. We will describe the method development for analysis of antisense oligonucleotides (ASO), silencing RNA (siRNA), short guide RNA (sgRNA) and messenger RNA (mRNA). In addition, we will illustrate the impact of non-specific interactions with LC metal hardware on oligonucleotide quantitation, tailing, and carryover. Click here for more details.
Supercritical Fluid Chromatography (SFC): A Powerful & Greener Tool for Analytical and Preparative Separations
November 17; 8:30am – 5:00pm
Instructors: Yingru Zhang, Lotus Separations & Mike Hicks, Merck & Co.
Course Description:
SFC is becoming a mainstream technique in recent years for its complementarity to HPLC with orthogonal selectivity, enhanced speed and efficiency, and lower solvent usage, thus “greener”. This course not only introduces SFC theories but also teaches SFC method development with practical instructions on successful implementation of SFC as an everyday separation technique to compliment other chromatography. First, we cover SFC principals necessary to develop successful SFC methods taking participants step by step through the instrument configuration and setup, column and mobile phase selections, and SFC parameters optimization for both chiral and achiral applications. Second, we will guide the participants through physical properties and chromatography attributes to understand the power and potential of SFC. Finally, we cover the intrinsic advantages of modern SFC technology and its expansion to a broader spectrum of SFC applications for more experienced chromatographers. SFC applications in pharmaceuticals, food chemistry, cannabis, lipids, and peptides will be discussed. Click here for more details.
Getting the most from GC and GC/MS
November 19; 8:30am – 5:00pm
Instructors: Gregory Slack, Consultant & Nicholas Snow, Seton Hall University
Course Description:
Gas chromatography (GC) and GC/mass spectrometry (MS) are staple techniques in chemical analysis. This course will enable users of GC and GC/MS to extend the power of their instruments to separate wider ranges of compounds with better sensitivity and fewer problems. Specific sessions will focus on method optimization for speed, sensitivity and/or ease-of-use, instrument set-up and qualification, troubleshooting and maintenance and effective combination of sampling, sample preparation, separation and detection to maximize method performance. Attendees are encouraged to bring example problems from their own work for discussion.
Click here for more details.
High-Performance Thin-Layer Chromatography a Reliable Analytical Technique in a Quality Control Environment
November 17 and 18; 8:30am – 5:00pm
Instructors: James Kababick, Flora Research Laboratories & Wilmer Perera, CAMAG Scientific
Course Description:
High-performance thin-layer chromatography (HPTLC) has been widely used in the dietary supplements industry and it’s become the gold standard for the identification of botanicals. The HPTLC features together with the comprehensive fingerprinting offers a complementary approach to describing the quality of complex samples in a unique way. HPTLC is also a powerful tool to perform quantitative analysis, hence, it can be used as a reliable analytical technique in any application field. This 2-day short course will cover concepts, parameters and unique features of HPTLC. It will discuss the rigorous standardization of the technique and how combined with full automation and modern approaches can be applied to routine quality control analysis. The use of case studies will emphasize the advantages of the technique and the practical sessions highlight strategies for developing and validating qualitative and quantitative methods. Attendees will have the opportunity to discuss current concepts and the future of HPTLC in an open forum.
Click here for more details.
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