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COURSE DESCRIPTION
High-performance thin-layer chromatography (HPTLC) has become widely accepted as reliable analytical method for identification of botanicals in a cGxP environment. It is also an established quantitative tool in various fields of application. The unique features of HPTLC fingerprints and the new concept of comprehensive HPTLC fingerprinting offer complementary and orthogonal approaches to describing the quality of complex samples. Now, multiple fingerprints from complementary chromatographic systems and multiple detection modes based on fully automatic analyses are realty and could transfer HPTLC into a new technique of instrumental analysis applied to routine quality control.
This course will start with a brief discussion of concepts, parameters and unique features of standard HPTLC. Then, it will explore new possibilities that arise from rigorous standardization combined with full automation and modern approaches to intelligent data evaluation. Interactive case studies examine and highlight the advantages of the technique. Practical sessions emphasize strategies for developing, fine-tuning, and validating qualitative and quantitative HPTLC methods. An open forum will provide opportunities to discuss new concepts and arising possibilities.
WHO SHOULD ATTEND
Researchers, Scientists, Academicians, QA/QC/R&D Personnel, Lab Managers and Technicians
Anyone wanting to learn about the extraordinary capabilities of HPTLC.
Anyone wanting to learn about how to get the most of an existing HPTLC system.
Anyone who wants to start thinking out of the box of liquid chromatography
TOPICS
1. Concepts, Parameters, and Special Features of HPTLC
a. What is so special about HP-TLC?
b. Rigorous standardization and flexible hyphenation – the two strong elements of HPTLC
c. Automatic HPTLC – the new basis for new analytical approaches
2. Practical Session: Generating Reliable HPTLC Data
3. Comprehensive HPTLC Fingerprinting
a. The Universal HPTLC mix in system suitability testing
b. Comprehensive HPTLC Fingerprinting: a different look at quality
c. Complementary HPTLC fingerprints
4. Case Studies: HPTLC in Practice
5. Quantitative Analysis by HPTLC
a. Principles of scanning densitometry
b. Assays and limit tests
c. Economic considerations
6. Practical Session: Development and Validation of HPTLC Methods
7. The Future of HPTLC
a. Specialized vs. universal screening methods
b. Complementary mobile phases for routine quality control
c. “New” definitions of quality
8. Open forum: questions and answers about the future of HPTLC
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