We're always looking for new lab tools to make protocols easier and results better. Our partner, Nepa Gene, has recently introduced the novel TiYO Autofluorescence Quenching System for labs in need of sharper, more precise fluorescent signals from cells and tissue sections. Early results are in and the TiYO is delivering as promised. Read more below.

The TiYO Autofluorescence Quenching System is a new type of instrument designed to solve a common problem in labs performing microscopy. Often, cells and tissues exhibit natural autofluorescence that can impede analysis with fluorophore markers, whether antibody-based or direct stains. The TiYO has perfected a simple concept -- photo-bleach slide sections, floating sections, and whole mounts before staining so that background signal is markedly reduced.

The key to this successful design lies in broad-wavelength LEDs coupled with evaporative cooling, ensuring the sample's molecular structure is not altered during the quenching process. Our early adopters agree that it's a winning combination and helps simplify later analyses of tissue sections after staining. If your lab has used Everbrite TrueBlack^TM reagent to help solve the autofluorescence riddle, you'll find the TiYO results are cleaner and with no secondary effects.

As the TiYO is a brand new product, we are offering free onsite evaluations for qualified labs. And only for readers of The Barker, use coupon code BDB2406TIYO to receive an additional $500 discount when ordering online or requesting a quote. We know you'll be impressed with the value that the TiYO will bring to your fluorescence microscopy experiment. [Valid for customers in the USA and Canada only. Introductory price of $4,495 ends July 1, 2024.][Everbrite TrueBlack™ is owned and sold by Biotium Corporation. Bulldog Bio is not affiliated with Biotium and does not sell or distribute Everbrite TrueBlack reagents.]

Advantages:

• EFFECTIVE | Significantly reduces background autofluorescence
• VERSATILE | works with all types of tissue and sections
• FAST | photobleach in under 2 hours

The TiYO Autofluorescence Quenching System has been shipping to labs throughout North America for the past 4 months. Their feedback has been remarkably positive and we've included a few images and quotes to help give you a sense of its capabilities.

Our line of TaqDog PCR reagents is growing! We want to keep the momentum going, so we are tickled green to offer our 2x Green Master Mixes for as little as 1-penny per reaction. That's right! Our TaqDog Premium 2X Green Master Mix is $2 for 200 reactions. And our TaqDog Hot Start 2x Green Master Mix is only $7 for 200 reactions. Both have reaction sizes of 50 µl providing your lab with ample amplification power. Use Discount Code BDB2406TDMM to receive this fantastic offer. [Valid for customers in the USA and Canada only. Discount only applies to 1 item either TDPM020 of TDHM020 (5 mL bottle for 200 x 50ul rxns). Offer ends July 1, 2024.]

Customer Feedback:

“We switched to TaqDog Hot Start mastermix more than a year ago. Previously, we used polymerases from other vendors, but TaqDog outperformed other, much more expensive mastermixes. We run hundreds of PCRs per week on complicated, very degraded templates – and robustness of PCR reagents is very important for us.”

Oleksandr, TruMe Labs, 2024



“The TaqDog mastermix has performed very well within our lab’s molecular workflow. We previously used a competitor’s Taq polymerase, which is much more expensive than the TaqDog polymerase. We’ve enjoyed using your product in PCRs, and we look forward to restocking our supply with TaqDog!”

Matt, ASU, 2024

“TaqDog Hot Start 2X Green Master Mix is a convenient and cost-effective option we use for routine PCR screening in high-GC mycobacteria. It has performed well for us with a variety of template preps and amplicons.”

Brian, Trudeau Institute, 2024

“Happy to report that your 2x master mix worked quite well in my hands. I am always looking for ways to improve my throughput and reduce waste.”

Chris, Stonehouse Breeding 2024

“We have used the TaqDog mastermix … and I’m very happy with it. It definitely simplifies the PCR setup and cuts down on time and opportunities for mistakes by having everything already in a mastermix. I’d say it seems comparable to [competitor product] that we used previously in terms of results, but TaqDog has multiple advantages of being a mastermix, hotstart, and preloaded with dye. … all things considered I am extremely pleased with TaqDog.”

Ben, Athanor Biosciences 2023